Abstract
This study aimed toevaluate the immunomodulatory effect ofthe probiotic Limosilactobacillus reuteri L26 BiocenolTM (L26) and its purified exopolysaccharide (EPS) with respect toantiviral innate immune response. Inour experiment, weused porcine epithelial IPEC-J2 cells asamodel ofthe intestinal barrier inahomologous infection byporcine RotavirusAstrain OSU6 (RVA). The production ofselected molecules ofnon-specific humoral immunity was evaluated atthe mRNA level. The EPS alone significantly increased the level ofinterferon λ3 (IFN-λ3) mRNA inthe non-infected IPEC-J2 cells (P<0.001). Wealso tested whether the treatment ofIPEC-J2 cells byL26 orEPS influences the replication ofRVA byvirus titration and real-time PCR. Wefound that apre-treatment incombination with subsequent continuous stimulation has noinfluence onthe RVA replication. However, both treatments significantly decreased the RVA-induced production ofIFN-λ3 (P<0.05) and the "SOS" cytokine interleukin6 (IL-6; P<0.01), already atthe transcription level. Inaddition, the EPS treatment resulted insignificantly increased IL-10 mRNA inthe RVA-infected cells. Insummary, weassume animmunoregulatory potential ofL.reuteri L26 BiocenolTM and its EPS inthe local intestinal antiviral immune response.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
