Abstract
Human embryonic diploid fibroblast cultures (WI-38) were maintained for periods of 3 and 5 weeks in a stationary phase by low (0.1 percent) serum medium. Upon subcultivation, these cultures underwent fewer cell doublings than continuously subcultivated controls. All the cultures reached the degenerative Phase III state at approximately the same calendar time regardless of the number of cell doublings. Limited culture lifespan of WI-38 may not be directly related to cell doubling potential, as previously reported, but may be a function of total metabolic time in vitro.
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