Abstract
Lignin peroxidase belongs to ligninolytic enzyme group and is one of the industrial important enzymes as it has wide applications in different sectors. Lignin peroxidase is produced by submerged fermentation process which requires optimization of physical and chemical parameters to achieve higher activity and make the process cost effective. The present study aimed at the optimization of physical as well chemical parameters of production medium. The optimization includes physical parameter such as incubation time, inoculum size, temperature, pH, RPM (Rotation per minute) while chemical parameters include carbon source, nitrogen source and different mineral elements. Form the optimization study, it was observed that highest lignin peroxidase production was achieved after 72 hours of incubation at temperature 300C, pH 6 and RPM 120. Optimization of chemical parameters reveals that incorporation of sodium nitrite (9g/L) in the media gave significant increase in enzyme activity. It was found that the maximum productivity achieved after optimization was 2214 U/ml which was four times higher than process without optimized parameters.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
