Abstract
The main objectives of this study were to optimize the culture conditions for theproduction of lignin peroxidase by Ganoderma leucidum, economic utilization of waste corn cobs as inducers substrate by pollution free fermentation technology and to optimize the solid state fermentation (SSF) process for lignin peroxidase (LiP) production. Growth medium employed for the culture of G. leucidum for the production of LiP was supplied with fermentation conditions that were optimized before selection like incubation period, inoculum size, temperature, pH, substrate to water ratio, nitrogen source, yeast extract and cane molasses. Culture was harvested on the fourth day and diluted five times with distilled water and filtrate was stored in Eppendoff tubes for enzyme assay using 310 nm wave lengths in the spectrophotometer. Lignin peroxidase production was enhanced and maximum LiP activity (2807 U/ml) was found in the growth medium after 96 h, inoculum size 3 ml, pH 4.5 and temperature 35°C with substrate to water ratio of 20 ml/5 g, yeast extract 4%, (NH4)2SO4, 2% and cane molasses 3%. Results indicate the excellent scope of corn cobs as solid state substrate for the production of lignin peroxidase by G. leucidum. Key words: Lignin peroxidase (LiP), Ganoderma leucidum, fermentation, corn cobs, cane molasses.
Highlights
Lignin is the most abundant natural aromatic polymer in the biosphere
Growth medium employed for the culture of G. leucidum for the production of lignin peroxidase (LiP) was supplied with fermentation conditions that were optimized before selection like incubation period, inoculum size, temperature, pH, substrate to water ratio, nitrogen source, yeast extract and cane molasses
Lignin peroxidase is an enzyme which is potentially useful for processing of renewable resources in the pulp and paper industry such as ligniocellulosics, as well as for enzymatic transformations of polyaromatic hydrocarbons
Summary
Lignin is the most abundant natural aromatic polymer in the biosphere. Lignin makes the second most abundant group of biopolymers in the biosphere; its biodegradation is an Abbreviations: LiP, Lignin peroxidase; SSF, solid state fermentation. Studies of lignin biodegradation is of great importance for possible biotechnological applications, as lignin polymers are a major obstacle to the efficient utilization of cellulosic materials in a number of industrial processes. Most commonly used WRF in early studies was Phanerochaete chrysosporium. It mostly produces two specific peroxidases, lignin peroxidase (LiP) and manganese peroxidase (MnP), other WRF secrete variable sets of enzymes include laccase and versatile peroxidase (VP) (Claudia et al, 1996; Asgher et al, 2006a)
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