Abstract
The article deals with the concentration changes and relative content of bacterial cells of Desulfuromonas acetoxidans in the intervals of sizes 0.2–2.0 µm under the influence of nano silver particles. Correlation between these changes of light-scattering properties of bacterial cells and growth abilities of bacteria Desulfuromonas acetoxidans under inflence of silver nanoparticles and ions has been shown. The purpose of the work was to research the intensity of processes the change of indexes of the antioxidant system the cells of Desulfuromonas acetoxidans at influence of silver nanoparticles and silver nitrate. The influence of various concentrations of silver nanoparticles and silver nitrate on enzymatic activity of catalase and reduced glutathione synthesis by Desulfuromonas аcetoxidans cells under their cultivation with fumarate addition and with absence of sulphur has been determined. Specific catalase activity increased with enhancing of concentration and duration of bacterial cultivation under the addition of this salt. The highest specific catalase activity was determined on the second day of bacterial growth under the influence of all concentration range of investigated metal salt. The reduced glutathione content under silver nitrate and silver nanoparticles exposure varied depending on the cultivation time and metal concentration. The maximum reduced glutathione content has been observed. The result of catalase activity changes and glutathione content changes of sulfur-reducing D. acetoxidans bacteria cell-free extracts and has been investigated under the influence of different concentrations of Ag nanoparticles during four days of cultivation has been investigated.
Highlights
Desulfuromonas acetoxidans are colorless strictly anaerobic sulfurbacteria that support reductive stage of sulfur cycle in the nature
Possess mainly a respiratory type of metabolism with elemental sulfur serving as the terminal electron acceptor, and being reduced to H2S. l-Malate or fumarate may be fermented, giving succinate as the main product in the presence or absence of acetate
For researches bacterial cells Desulfuromonas acetoxidans IMB B-7384 strain was isolated from Yavoriv aquatic sulfur field (Lviv region, Ukraine)
Summary
Desulfuromonas acetoxidans are colorless strictly anaerobic sulfurbacteria that support reductive stage of sulfur cycle in the nature. In Bergey's Manual of Systematic Bacteriology they are described as “straight or slightly curved rods and elongated ovoid rods, 0.4–0.9 – 1.0–4.0 mkm in length. Special resting forms such as spores are not known to occur. For researches bacterial cells Desulfuromonas acetoxidans IMB B-7384 strain was isolated from Yavoriv aquatic sulfur field (Lviv region, Ukraine). It was purified and identified at the Department of Microbiology of Ivan Franko National University of Lviv, Ukraine.
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