Abstract
Silver Nanoparticles were synthesized by Esche- richia coli using Silver nitrate in the growth me-dium and characterized in X-Ray Diffraction, UV-Vis Spectrophotometer and Scanning Electron Microscope. They exhibited antimicrobial activity against human pathogens except Escherichia Coli. Nanoparticles were impregnated in yarn and ana-lyzed for their inhibition in the broth culture. The Minimal Inhibitory Concentratio was calculated for the human pathogens in Microtitre plate. The toxicity assessment of the nanoparticles in the embry-onic Zebrafish showed many organogensis deformi-ties like cardiac malformations, eye and head edema, tail and trunk flexure were observed in the organ system of the developing embryos for 1 to 5 day post fertilization in different concentrations of Ag Nanoparticles. The Organogenesis disruptive effects were found in 14 - 20 ng/ml of silver nanoparticles but the inhibition was found in 4-10ng/ml for the pathogens in vitro and 10ng/ml in embryos. Nevertheless, in Cardiac assay, the Heart Beat rates were calculated as 42 - 45 for 15 Sec in the concentrations ranging from 10 - 20 ng/ml of Silver nanoparticles. The blood flows, rhythmicity, contractility of heart beat rates were observed normal. The Mean value of blood Cell counting did not showed any notable effects in the Nanoparticle treated Zebrafish embryos and control. The LC50 value for the Biosynthesized nanoparticle was at 22 ng/ml in all the developmental stages of the em-bryos. Our results shows silver nanoparticles dis-rupts the normal organogenesis during development and further detailed studies are needed to prove silver nanopartcles are an antimicrobial agent for use in humans.
Highlights
Microorganisms such as bacteria, yeast and fungi play an important role in remediation of toxic metals through reduction of metal ions; this is considered interesting as nanofactories [1]
Our results shows silver nanoparticles disrupts the normal organogenesis during development and further detailed studies are needed to prove silver nanopartcles are an antimicrobial agent for use in humans
All diffraction peaks correspond to the characteristic face centered cubic (FCC) silver lines
Summary
Microorganisms such as bacteria, yeast and fungi play an important role in remediation of toxic metals through reduction of metal ions; this is considered interesting as nanofactories [1]. Nanoparticles has a significant potential for a wide range of biological applications such as antifungal agents, antibacterial agents for antibiotic resistant bacteria, preventing infections, healing wounds and anti-inflammatory [2]. The new technology of impregnation of silver nanoparticles are commercially available and silver coated dressings are used extensively for wound management, in burn wounds [3,4], Chronic leg ulcers [5], diabetic wounds [6] and traumatic injuries. The interactions of silver nanoparticles with biosystems are just beginning to be understood, and these particles are increasingly being used as microbicidal agents. A new generation of dressing with antimicrobial agents like silver and biopolymer was developed to reduce or prevent infections
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