Abstract
The effect of subculture and temperature on shoot differentiation of rice callus was examined. The callus tissues were cultured in the medium containing kinetin. Starch granules and other cell constituents of the callus were observed in their green regions. While rooting capacity in Te-Tep remained in long term callus cultures over 15th passage, any shooting was not obtained in Aichi asahi cultures over 9th passage. Variation in temperature condition was effective in callus growth, but not in shoot differentiation. Starch granules were stained purple or reddish purple colour with Lugol's solution. In addition, large granules of dark purple colour were observed in green regions of callus tissues in kinetin medium. Disappearance of the starch granules were observed in the tissues in gibberellin medium after 9 days culture. A technique was investigated for epon-sections of rice callus tissues. Some staining methods of azure II·methylene blue, fuchsine-methylene blue, hematoxylin-safranin and toluidine blue were used. Nuclei, nucleoli, osmiophilic granules and cell walls were observed in the green region. Especially, morphological characteristics were revealed in chloroplasts, starch granules and amyloplasts.
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