Abstract
The purpose of this study was to describe of some histological structures and histochemical features of the absorptive cells and goblet cells in intestine of common carp. In this study, Fifteen adult male common carp were catching alive from the AL-Forat river, with age about (7 - 12) months and mean of their weight was (2100 ± 81 g) and mean standard length was (50.4 ± 3.1 cm), immediately after death.Incision was made through the midventral line of the fish just from cranial to the anus to expose the intestine, specimens of intestine were taken and washed with (0.9 %) normal saline solution. Ten samples were obtained from different regions of each portion of the intestine (anterior, middle and posterior), fixed by either 10% neutral buffered formalin or Bouin’s solution approximately 24 hours at room temperature and then treated by routine histological processing. The stains were used, Hematoxylin and Eosin, periodic acid Schiff, Alcian blue pH 2.5, and Combinated Alcian blue PH 2.5 plus periodic acid Schiff. The Mean number, height and width of mucosal folds were measured and counts of goblet cells in the mucosal folds of each portion of the intestine. The results showed that the intestinal epithelium is simple columnar, the enterocytes were tall columnar cells with brush border; goblet cells were ovoid, located between the enterocytes. The folds composed of the entire thickness of the mucosa, mean number, height and width of the mucosal folds were few in posterior portion of intestine compare with that in other portions. The anterior portion of the intestine has largest number of mucosal folds, while has less number of goblet cells compare to middle and posterior portions of the intestine. The epithelial cells stained red-purple by periodic acid Schiff stain with Alcian blue, the goblet cells were take positive reaction with periodic acid Schiff stain, and with Alcian blue plus periodic acid Schiff, while negative with only Alcian blue stain. Glycoprotein staining intensity varied from very weak to moderate, whereby the presence of neutral, acidic and sulphatedglycoconjugates. In conclusion, numbers of goblet cells were observed to be increased towards posterior portion of intestine required as lubricant for fecal expulsion, and reversed relation between number of the folds and goblet cells.
Highlights
The family Cyprinidae, commonly known as cyprinids, is the largest of all fish families found throughout the world; members of the family have a wide geographical distribution and occur in Europe, Asia, Africa, and North America.There are about 2900 species of cyprinids worldwide [1]
As in other vertebrates, the digestive tract consists of the alimentary canal, a hollow tube of varying diameter which is divided into the esophagus, stomach, intestines and rectum, some associated glands, salivary glands, liver, gall bladder, and pancreas [3]
The intestinal epithelium is made up of cells named enterocytes, goblet cells, lymphocytes, stem cells and enteroendocrine cells are scattered through the epithelium and rodlet cells are found in some species of teleost fishes [9,10,11]
Summary
The family Cyprinidae, commonly known as cyprinids, is the largest of all fish families found throughout the world; members of the family have a wide geographical distribution and occur in Europe, Asia, Africa, and North America.There are about 2900 species of cyprinids worldwide [1]. The intestinal epithelium is made up of cells named enterocytes, goblet cells, lymphocytes, stem cells and enteroendocrine cells are scattered through the epithelium and rodlet cells are found in some species of teleost fishes [9,10,11]. The columnar epithelial cells that dominate the intestinal epithelium and possessing microvilli, the enterocytes in the hindgut of fish have an antigen-transporting capability and many macrophages and lymphoid cells are distributed among the epithelial cells and in the lamina propria. Goblet cells derive their name from challislike shape. The nucleus is located in the tapered stem, which widens and constricts to from an apical pore through which mucus is discharged, goblet cells are common components of the intestinal mucosa in fish [12,13,14]
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