Light field microscope with square aperture and the reconstruction algorithm

Abstract

Light field microscope (LFM) can be built by inserting microlens array into a traditional microscope. Different perspective image of the sample could be obtained by reconstruction algorithm dealing with the four dimensional light field information captured by the detector. Since light field microscope has been always studied in laboratory, the reconstructed image quality which depends on the microlens array is still not too high. The pixels between every two sub image of microlens array is waste when circle aperture of objective is used. In this article, rectangular aperture has been used in this paper to improve the utilization of the CCD, which is favorable to reconstruction algorithm. Progress has been made in reconstruction algorithm which can improve the reconstruction image quality, wave optic theory has been used to calculate the PSF of the whole imaging system due to the short object distance, subpixel interpolation algorithm has been combined with the reconstruction algorithm of LFM to improve the image quality, good imaging quality has been obtained through the improved algorithm.