Abstract
Light-microscopic study of muscles from 8 patients with Duchenne muscular dystrophy (DMD) and 9 control cases transplanted into nude mice showed the formation of cross-striations and peripheral nuclei in regenerating muscle fibers of both DMD and control grafts up to 4 weeks. Quantitative study of the diameter of myotubes and myoblasts in grafted muscles showed that the group mean diameters of DMD myotubes and myoblasts were 13.4 ± 0.8 (SE) μm, 15.6 ± 0.7 μm, 17.1 ± 0.9 μm and 16.2 ± 1.0 μm after 1, 2, 3 and 4 weeks of transplantation, respectively, whereas those of control myotubes and myoblasts were 14.9 ± 0.6 (SE) μm, 19.9 ± 0.5 μm, 21.3 ± 0.7 μm and 20.7 ± 0.7 μm after the same intervals. The group mean diameters of the DMD regenerating myotubes and myoblasts were significantly smaller than those of control myotubes and myoblasts at 2 ( P <0.001), 3 ( P <0.005) and 4 ( P <0.005) weeks after transplantation. Electron-microscopic findings of DMD muscle grafts were morphologically similar to those seen in control muscle grafts at all stages of this experiment.
Published Version
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