Abstract
In order to perform strain improvement of the methanol utilising yeasts Pichia pinus and Pichia lindneri their life cycles were elucidated. Both yeasts are monoecious and self-fertile. Karyogamy by autodiploidization is followed by meiosis leading to Mendelian segregation within the offspring. In Pichia pinus crosses via cell or protoplast fusion is feasible as proven by tetrad analysis. This is not the case in Pichia lindneri. The only possiblity to obtain recombinants in this yeast is brought about by a yet unknown mechanism, leading to a unilateral transfer of genetic material, found after cocultivation of mutants. This is revealed by the evaluation of F1 offspring from meiotic divisions after autodiploidisation. Although analysis of nuclear and mitochondrial DNA revealed rather gross differences not only between the species but also within different strains of Pichia pinus, only a limited exchange of genetic material is possible in interstrain crosses.
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