LEVELS OF CERTAIN HORMONES AND RECEPTORS IN TUMOR AND PERITUMORAL AREA IN BALB/C NUDE FEMALE MICE WITH MULTIPLE PRIMARY MALIGNANT PROCESS DEVELOPING ON THE BACKGROUND OF PRIMARY IMMUNODEFICIENCY

Abstract

Primary immunodeficiency is one of the reasons for the development of primary multiple malignant tumors.
 The aim of the study is to examine the levels of certain hormones and their receptors in the tissue and peritumoral area of B16/F10 melanoma and Lewis lung carcinoma (LLC) in case of isolated and combined subcutaneous growth in female mice with T-cell immunodeficiency.
 Materials and Methods. BALB/c Nude mice were divided into groups: intact group; 2 control groups (Group 1 with B16/F10 subcutaneous inoculation, Group 2 with LLC subcutaneous inoculation); main group (animals with LLC+B16/F10 inoculation). The ELISA method was used to determine the levels of free testosterone (Tf.), estrone (E1), prolactin (PRL), estrogen receptors (REα and REβ), androgen receptors (RA) and progesterone receptors (RP4) (Cassabio, China). Statistical processing of the obtained results was carried out on a personal computer using STATISTICA 10.0, parametric Student's test and nonparametric Wilcoxon-Mann-Whitney test.
 Results. In the main group, life expectancy reduced due to melanoma growth by 1.8 times and LLC decrease by 2.3 times. In animals with LLC+B16/F10, compared to those with an only one tumor growth variant, estrone level in tumors decreased, but free testosterone level increased. Melanoma growth in animals with LLC+B16/F10 was accompanied by an increase in prolactin level and some sex steroid receptors in the tumor tissue, its peritumoral area, and skin not affected by the malignant process. A decrease of Lewis carcinoma in females of the main group was accompanied by a decrease of all the studied receptors and hormones both in the tumor and its peritumoral area.
 Conclusions. Melanoma growth is probably associated with a high prolactin level and sex steroid receptors in unaffected skin, being a source of melanocytes, the cells from which this tumor develops.