Abstract
This article aimed at discussing the effectiveness of developing a new antioxidant and anti-inflammatory product from the natural source namely the extract of Leucas aspera plant that can be used to treat various oral mucosal conditions. The study was a descriptive experimental study. The dried powder extract of the whole LA plant was subjected to various procedures such as phytochemical analysis, antioxidant assay (DPPH) and assessment of anti-inflammatory effect (Cytokine activity in RAW 264.7 macrophage cells) and the results were tabulated. Significance of anti-inflammatory activity was tested by T-test. Our analysis has confirmed that 100µg of plant extract showed high amount of antioxidant activity (78%). The cytokine inhibitory percentage of our plant extract showed consistent increase when tested from 250 ug/ml to 1000ug/ml to be from 24% to 39% thus demonstrating significantly high level of anti-inflammatory potential (P > 0.01). Our study suggests that LA plant can be safely utilized for testing and developing products based on its extract.
Highlights
The existence of widespread nutritional deficiencies and tobacco related habits makes occurrence of aphthous ulcers and premalignant lesions such as oral leukoplakia, erythroplakia, lichen planus, actinic cheilitis, submucous fibrosis as well as palatal lesions in reverse smokers are more common among the Indian population
Phytochemical Analysis The obtained extract was run through various phytochemical analysis and the results were tabulated. 10mg of plant extract is dissolved in 10ml of methanol(1mg/ml) and 500ul extract was used for each reaction
The plant extract subjected to various procedures such as phytochemical analysis, Antioxidant assay (DPPH) and Anti-Inflammatory properties assay (Cytokine activity in RAW 264.7 macrophage cells) have been reported as follows: Phytochemical Analysis The 500ul plant extract was subjected to tests for carbohydrates, flavonoids, sterols, alkaloids, glycosides, saponin, phenolic compounds and sterols
Summary
The existence of widespread nutritional deficiencies and tobacco related habits makes occurrence of aphthous ulcers and premalignant lesions such as oral leukoplakia, erythroplakia, lichen planus, actinic cheilitis, submucous fibrosis as well as palatal lesions in reverse smokers are more common among the Indian population. To their potent pharmacological activities, lower toxicities and economic viability.[1] The roots and stems of leucas aspera (LA) has demonstrated high antioxidant activity[2] in comparison to vitamin E and can be included as an active formula in treating such potentially malignant disorders. The anti-inflammatory property of leucas aspera would be beneficial in treatment of aphthous ulcers and stomatitis. Leucas is an ideal extract to treat the most common oral disorders as a whole; based on its antioxidant and anti-inflammatory efficiency. It can be considered as a potential substrate to develop all-purpose medication for oral topical usage
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