Letter to the editor] The selenium content of cell culture serum influences redox-regulated gene expression

Abstract

Cell Culture The MDA-MB-231 breast cancer cell line (see Reference 24 in the main text) was cultured in RPMI 1640 medium (Invitrogen, Melbourne, Australia), supplemented with either 10% FBS [either Invitrogen, Lonza (Melbourne, Australia), Quantum (Brisbane, Australia), or 10% serum supreme (Lonza) and 100 μg/mL penicillin (Invitrogen) and 100 μg/mL streptomycin (Invitrogen) in 5% CO2 at 37°C. Media used in transfections did not contain penicillin or streptomycin. All chemicals were purchased from SigmaAldrich (Castle Hill, Australia) unless otherwise specified.