Abstract
Objective: to study an association of leptin (LEP) A19G polymorphism and leptin receptor (LEPR) Gln223Arg AND Lys109Arg polymorphisms with the predilection for postmenopausal osteoporosis (OP). Subjects and methods. PCR analysis was used to examine the polymorphisms among 428 women (254 patients with OP and 174 healthy women). The anthropometric, densitometric, and biochemical markers of bone remodeling and standard clinical and biochemical parameters were studied. Results. Statistically significant differences were found in the distribution of the genotypes of LEP A19G polymorphism between the women with OP and the controls (χ2 = 9.41; p = 0.009). In the patients with OP, the 19GG genotype frequency was significantly higher than that in the controls [OR = 2.0; 95% confidence interval (CI) 1.13-3.52 (p = 0.011)]. LEP 19GG genotype carriers were found to have lower mineral bone density (MBD) of the femoral neck than heterozygotes (p = 0.06). In LEPR 223GlnArg heterozygotes, the mean MBD of the trochanter and whole hip was statistically significantly lower than that in patients with the genotype 223ArgArg (p = 0.013). 223GlnGln carriers were taller than 223GlnArg ones (p = 0.04). There were no associations of the clinical and biochemical parameters with the polymorphisms studied. Conclusion. Our study confirmed the role of LEP A19G and LEPR Gln223Arg polymorphisms as important candidate genes involved in the formation of a predilection for OP.
Highlights
PCR analysis was used to examine the polymorphisms among 428 women (254 patients with OP and 174 healthy women)
Significant differences were found in the distribution of the genotypes of LEP A19G polymorphism between the women with OP and the controls (χ2 = 9.41; p = 0.009)
In leptin receptor (LEPR) 223GlnArg heterozygotes, the mean mineral bone density (MBD) of the trochanter and whole hip was statistically significantly lower than that in patients with the genotype 223ArgArg (p = 0.013). 223GlnGln carriers were taller than 223GlnArg ones (p = 0.04)
Summary
Учреждение Российской академии медицинских наук Научно-исследовательский институт ревматологии РАМН, Москва. Исследовать связь полиморфизма A19G гена лептина (LEP) и полиморфизмов Gln223Arg и Lys109Arg гена рецептора лептина (LEPR) c предрасположенностью к постменопаузальному остеопорозу (ОП). Выявлена сниженная минеральная плотность костной ткани (МПКТ) шейки бедра у носителей 19GG-генотипа гена LEP по сравнению с гетерозиготным генотипом (p=0,06). У носителей гетерозиготного 223GlnArg-генотипа гена LEPR средние показатели МПКТ трохантера и всего бедра были статистически достоверно ниже, чем у пациенток с 223ArgArg-генотипом (p=0,013). Objective: to study an association of leptin (LEP) A19G polymorphism and leptin receptor (LEPR) Gln223Arg AND Lys109Arg polymorphisms with the predilection for postmenopausal osteoporosis (OP). In LEPR 223GlnArg heterozygotes, the mean MBD of the trochanter and whole hip was statistically significantly lower than that in patients with the genotype 223ArgArg (p = 0.013). Our study confirmed the role of LEP A19G and LEPR Gln223Arg polymorphisms as important candidate genes involved in the formation of a predilection for OP. Специфические пары праймеров и рестрикционные эндонуклеазы, использованные для анализа полиморфизма длин рестрикционных фрагментов
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