Legacy effects of invasive grass impact soil microbes and native shrub growth

Abstract

AbstractIn California, invasive grasses have displaced native plants, transforming much of the endemic coastal sage scrub (CSS) to nonnative grasslands. This has occurred for several reasons, including increased competitive ability of invasive grasses and long-term alterations to the soil environment, called legacy effects. Despite the magnitude of this problem, however, it is not well understood how these legacy effects have altered the soil microbial community and, indirectly, native plant restoration. We assessed the microbial composition of soils collected from an uninvaded CSS community (uninvaded soil) and a nearby 10-ha site from which the invasive grass Harding grass (Phalaris aquaticaL.) was removed after 11 yr of growth (postinvasive soil). We also measured the survival rate, biomass, and length of three CSS species andP. aquaticagrown in both soil types (uninvaded and postinvasive). Our findings indicate thatP. aquaticamay create microbial legacy effects in the soil that likely cause soil conditions inhibitory to the survival rate, biomass, and length of coastal sagebrush, but not the other two native plant species. Specifically, coastal sagebrush growth was lower in the postinvasive soil, which had more Bacteroidetes, Proteobacteria,Agrobacterium,Bradyrhizobium,Rhizobium(R. leguminosarum),Candidatus koribacter,Candidatus solibacter, and rhizophilic arbuscular mycorrhizal fungi, and fewer Planctomycetes, Acidobacteria,Nitrospira, andRubrobactercompared with the uninvaded soil. Shifts in soil microbial community composition such as these can have important implications for restoration strategies in postinvasive sites.