Abstract
Lectins labelled with colloidal gold make useful cytochemical probes at the electron microscope level for identifying glycoconjugates in developing wheat grain. Gold-labelled wheat-germ lectin, which is specific for N-acetyl- D -glucosamine, and peanut Lectin (specific for D -galactose) label the storage protein bodies of the starchy endosperm and the cell walls of the nucellar epidermis. The labelling of these structures is completely inhibited by pretreatment of the sections with N,N´,N˝-triacetylchitotriose. The binding of gold-labelled peanut lectin is similarly inhibited by galactose. Gold-labelled wheat-germ and peanut lectins also bind to diffuse structures tentatively identified as rough endoplasmic reticulum, the likely site of storage protein synthesis.
Published Version
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