Abstract
-Histone A Tobacco plants subjected to methyl jasmonate treatment accumulate an N-acetylglucosamine (GlcNAc) binding lectin called Nicotiana tabacum agglutinin, shortly Nictaba (Chen et al. 2002). Purification and subsequent characterization of Nictaba showed that the lectin is a homodimeric protein composed of 19 kDa subunits. Using immunocytochemistry with a Nictaba-specific antibody it was shown that Nictaba accumulates in the nucleus and the cytoplasm of the parenchyma cells of jasmonate-treated tobacco leaves. These results were confirmed by confocal microscopy on tobacco plants transiently expressing the eGFP-labeled Nictaba protein (Figure 1).
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call