Abstract
Abstract Lectin and temperature dual-responsive glycosylated block copolymers, composed of a hydrophilic polymer backbone comprising poly(N-isopropylacrylamide) (PNIPAm) and polyacrylamide (PAAm), were synthesized by consecutive RAFT polymerization reactions. PNIPAm was synthesized by RAFT polymerization using a trithiocarbonate derivative for use as a macro chain transfer agent (CTA) in post-RAFT polymerization with a lactose-carrying acrylamide derivative used as a monomer substrate to obtain block copolymers. The resulting glycosylated block copolymers were responsive to temperature at around 33 °C (lower critical solution temperature; LCST) and formed aggregates 200 nm in diameter above the LCST in aqueous media. The aggregates specifically interacted with lectin in aqueous media above the LCST, forming precipitated conjugates. When the temperature was decreased below the LCST, the conjugate dissociated and dissolved into the aqueous medium. The conjugates composed of block copolymer and lectin are reversible and respond to changes in temperature.
Published Version
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