LC-MS/MS Screening of Phenolic Compounds in Wild and Cultivated Grapes Vitis amurensis Rupr.

Mayya Razgonova,Tatiana Kalenik,Irina Derbush,Yury Manakov,Konstantin Pikula,Evgeniy Kislin,Andrey Sabitov,Alexander Zakharenko,Sezai Ercisli,Kirill Golokhvast,Ivan Seryodkin

doi:10.3390/molecules26123650

Abstract

This work represents a comparative metabolomic study of extracts of wild grapes obtained from six different places in the Primorsky and Khabarovsk territories (Far East Russia) and extracts of grapes obtained from the collection of N.I. Vavilov All-Russian Institute of Plant Genetic Resources (St. Petersburg). The metabolome analysis was performed by liquid chromatography in combination with ion trap mass spectrometry. The results showed the presence of 118 compounds in ethanolic extracts of V. amurensis grapes. In addition, several metabolites were newly annotated in V. amurensis. The highest diversity of phenolic compounds was identified in the samples of the V. amurensis grape collected in the vicinity of Vyazemsky (Khabarovsk Territory) and the floodplain of the Arsenyevka River (Primorsky Territory), compared to the other wild samples and cultural grapes obtained in the collection of N.I. Vavilov All-Russian Institute of Plant Genetic Resources.

Highlights

The appearance of the first representatives of the Vitaceae family dates from the Upper Cretaceous period [1]
The diversity of phytochemicals identified in wild and cultural grape V. amurensis resulted in the following descending order: VZK (52) > ART (46) > SPB-2 (39) > SPB-1 (28) > SPB-4 (27) > PAK (25) > RIK (22) > KAL (20) > SPB-3 (19) > ARS (18)
The most diverse metabolome was identified in the grapes collected in the vicinity of Vyazemsky, Khabarovsk Territory, which was rich in flavanols and phenolic acids

Summary

Introduction

The appearance of the first representatives of the Vitaceae family (genus Vitis) dates from the Upper Cretaceous period [1]. Several types of fossil grapes of genus Vitis have been found in different parts of North America [2]. In the Eocene, representatives of the genus Vitis were widespread in Eurasia and the Far North [2]. Was found and described in the sediments of the Sakhalin Island, the Russian Far East. In the Paleogene, one of the best-preserved species of fossil grapes Vitis sachalinensis Krysht. These data show that the evolution of the vine in the territory of Russia proceeded from ancient times. There is very little information about the culture of East Asian grapes

Methods

Results

Conclusion