LC-MS/MS method for the simultaneous quantification of pyriproxyfen and bifenthrin and their dissipation kinetics under field conditions in chili and brinjal.

Abstract

Bifenthrin, a synthetic pyrethroid, and pyriproxyfen, a plant growth regulator, are used extensively in agriculture for controlling the different insect pests. The present study was undertaken to examine the dissipation behavior of a formulation with a combination of pyriproxyfen and bifenthrin on chili and brinjal under field conditions at four different locations. Dissipation study of combination of pyriproxyfen and bifenthrin revealed swift degradation in both crops with a half-life of 2.5-2.6 and 2.0-2.1 days in brinjal and chili, respectively. Also, a simple method for simultaneous quantification of pyriproxyfen and bifenthrin was developed and validated using modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) technique on liquid chromatography with tandem mass spectrometry (LC-MS/MS). Recovery of the method was found to be under an acceptable range of 90.0%-93.5% and 88.7%-94.3% in chili and 92.4%-96.6% and 97.4%-100.9% in brinjal for pyriproxyfen and bifenthrin, respectively. At harvest time, the terminal residues of bifenthrin and pyriproxyfen were below the maximum residue limits set by European Union in chili and brinjal, respectively, suggesting that the use of this pesticide formulation is safe and does not impose harmful effects on human health. PRACTICAL APPLICATION: In this paper, dissipation behavior of a pesticide formulation with a combination of pyriproxyfen and bifenthrin was undertaken under field conditions at four different locations on chili and brinjal in India. The simultaneous quantification of pyriproxyfen and bifenthrin using LC-MS/MS technique has been validated incorporating modified QuEChERS extraction method with limit of detection at 0.005µg/g and limit of quantification at 0.01µg/g, which is well below the EU-MRLs (European Union legislation Maximum Residue Level) of pyriproxyfen and bifenthrin in both chili and brinjal. Furthermore, dissipation kinetics of a formulation undertaken under field conditions at four different locations on chili and brinjal suggested that the terminal residues of both bifenthrin and pyriproxyfen were below the maximum residue limits set by European Union in chili and brinjal, respectively, at the time of harvest and that the use of this pesticide formulation is safe.