1.產後婦女使用生化湯與六個月時生活品質的關係 2.以極致液相層析/串聯式質譜儀分析尿液中五種鄰苯二甲酸酯類的代謝物:初步結果

Abstract

1. Abstract Background: Although Sheng-Hua-Tang (comprising Radix Angelicae Sinensis, Ligustici Rhizoma, Semen Persicae, Zingiberis Rhizoma and Glycyrrhizae Radix) use during the postpartum has been popular in Chinese communities over a long period, its benefits have not been evaluated in terms of its effects on the health-related quality of life of postpartum women. Objectives: This study aims to explore the relation between different patterns of Sheng-Hua-Tang use and the health-related quality of life in postpartum women. Design: A longitudinal birth cohort follow-up study. Settings and participants: We used multistage stratified systematic sampling to recruit 24,200 pairs, postpartum women and newborns, from the Taiwan national birth register in 2005. A structured questionnaire was successfully administered to 87.8% of the sampled population. Methods: Subjects underwent a home interview 6 months after their deliveries between June 2005 and July 2006. The Medical Outcomes Study 36-item Short-Form (SF-36) was used to measure the quality of life of the women with different patterns of Sheng-Hua-Tang use. Results: Compared with those who never used after delivery, the scores of role limitations due to physical health and emotional problems significantly increased in women who used Sheng-Hua-Tang within 1 month only but decreased in those who continuously used within 1 month and later. In addition, the scores of role limitations due to physical health and emotional problems significantly increased in women who used Sheng-Hua-Tang regardless of the frequency. Conclusions: Sheng-Hua-Tang use during the first month of the postpartum period may have a positive effect on women’s health-related quality of life especially in terms of role limitations due to physical health and emotional problems. However, continuous use after the first month of the postpartum period might have a negative effect on women’s quality of life. Further studies are needed to replicate the results and elucidate the causal relations. 2. Abstract Background: Phthalates are widely used as plasticizers in industry and our daily life and their hazards of reproductive and developmental dysfunction have been concerned. Validated analytical methods to measure the concentrations of phthalate metabolites in human are essential for assessing internal exposure to phthalates. Objectives: The main objective of this study is to modify the previously high performance liquid chromatography - tandem mass spectrometry (HPLC-MS/MS) analytical methods, further develop a speedy and sensitive analytical method to assess the five phthalate monoesters in human urine. Methods: The modified solid-phase extraction (SPE) procedure, enzymatic deconjugation technique, and isotope ultra-performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS) was used to analyze five phthalate monoesters, including monomethyl phthalate (MMP), monoethyl phthalate (MEP), mono butyl phthalate (MBP), monobenzyl phthalate (MBzP), and mono-(2-ethylhexyl) phthalate (MEHP) in urine. Results: The SPE recoveries of the five phthalate monoesters ranged from 89 % to 121 %, and the sample pretreatment recoveries ranged from 79-105%. Ion enhancement effect was founded in MMP and MEP, ion suppression effect of MBP, MBzP and MEHP was 18 %, 20% and 68 %, respectively. Instrumental detection limits (IDLs) ranged from 0.36 pg to 11.8 pg and instrumental quantitation limits (IQLs) ranged from 1.19 pg to 39.3 pg, respectively. The IDL and IQL were lowest in MEHP, and highest in MMP. Conclusions: The modified sample preparation processes maintain good SPE and sample pretreatment recoveries. And we developed a rapid analytical method for determination of five phthalate metabolites using isotopic UPLC-MS/MS due to the chromatographic time was only 6.7 min per run. The limit of detection (LOD), limit of quantification (LOQ), accuracy and precision of the method are still needed to calculate to manifest it is also a sensitive and accurate method.