Abstract
This work aimed to ascertain the phenolic compounds and assess the antioxidant capacity and alpha-glucosidase inhibitory activity of Centaurea papposa extracts. Phenolic compounds were appraised using LC-MS technique. Moreover, antioxidant activity was investigated using DPPH, ABTS, CUPRAC and FRAP assays. In vitro alpha-glucosidase inhibitory effect was carried out. LC-MS analysis revealed the presence of 21 compounds among which 13 were phenolic acids, 6 flavonoids, 1 phenolic aldehyde and 1 benzopyrone. The ethyl acetate extract exhibited the highest activity in ferric reducing antioxidant power (FRAP) assay (IC50: 22.9 ± 2.8 µg/mL). Nevertheless the n-butanol extract was the most active in cupric reducing antioxidant capacity assay (IC50: 3.1 ± 0.1 µg/mL). A significant alpha-glucosidase inhibitory activity was displayed by dichloromethane extract (IC50: 227.6 ± 4.4 µg/mL).
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 Cupric reduction antioxidant capacity: 1 min 47 sec: Full Screen Alternate
Highlights
Diabetes mellitus is a chronic disease of metabolic disorder (Pallavi et al, 2015) which is treated by either insulin or oral anti-diabetic drugs
Folin–Ciocalteu’s reagent (FCR), neocuproine, butylated hydroxytoluene (BHT), DPPH dye, sodium carbonate, aluminum chloride, phosphate buffer, ammonium acetate buffer, potassium ferricyanide, butyl hydroxyanisole (BHA), methanol and ethanol were obtained from Sigma Chemical Co., Germany. 2,20-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), p-nitrophenyl-a-D -glucopyranoside solution, alpha-glucosidase, ascorbic acid, tannic acid were obtained from Fluka Chemie, Germany
The LC-MS analysis of ethyl acetate extract revealed the presence of quinic acid, malic acid, gallic acid, protocatechuic acid, chlorogenic acid, vani
Summary
Diabetes mellitus is a chronic disease of metabolic disorder (Pallavi et al, 2015) which is treated by either insulin or oral anti-diabetic drugs. Among the oral antidiabetic drugs, alpha-glucosidase inhibitor retards the liberation of d-glucose from dietary complex carbohydrates and delays glucose absorption, resulting in reduced postprandial plasma glucose level and suppession of postprandial hyperglycemia. Plants such as Camellia sinensis (Hara and Honda, 1992), Ipomoea batatas (Matsui et al, 1996), berry (McDougall and Stewart, 2005), Syzygium zeylanicum, Cleistocalyx operculatus, Horsfieldia amygdalina and Careya arborea (Truong et al, 2007) show alpha-glucosidase inhibitor activity. Centaurea papposa is a species belonging to the Centaurea section, subsection Acrolophus (Cass.) DC (Hilpold et al, 2011), increasing wild in Algeria and Tunisia
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