Abstract
In this research, we aimed to combine the principles of herbal medicine and tissue engineering to develop a bioactive wound dressing. The nanofibrous scaffolds were produced via electrospinning of cellulose acetate/Lawsonia inermis extract (LIE) solution. The developed constructs were studied regarding their interaction with fibroblast cells and their various physicochemical and biological properties. The wound healing potential of the prepared dressings was studied on a rat model skin wound. In vitro studies showed that cellulose acetate scaffolds loaded with 3% LIE had the highest cell protection capacity under oxidative stress and promoted the fibroblast cells proliferation. Therefore, this group was used for wound healing assay. In vivo study showed that LIE-loaded wound dressings had the highest epithelial thickness, collagen deposition, and angiogenesis scores. Gene expression analysis showed that wounds treated with cellulose acetate/LIE wound dressings upregulated VEGF, b-FGF, and collagen type 2 genes. While, NFK-β and TNF-a genes expressions were significantly reduced by these dressings. This study implies that local delivery of LIE via cellulose acetate scaffolds can alleviate inflammation and increase the expression of pro-healing genes.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
