Abstract
A technique for lateral ow hybridization analysis of speci c bacterial beta-lactamase mRNAs has been developed, including the preparation of a biotinylated target DNA and its analysis on a test strip with immobilized oligonucleotide probes. When the DNA target and probe structures are complementary, DNA duplexes are formed in the test zone of the strip, which interact with the streptavidin conjugate with gold nanoparticles. The method was used to determine the transcripts of the TEM-type betalactamase genes isolated from the culture of E. coli - producers of the corresponding recombinant betalactamase. It has been shown that singlestranded DNA target of 330 bases was revealed most effectively in lateral ow analysis.
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