Abstract
BackgroundDefinitive endoderm (DE) gives rise to the respiratory apparatus and digestive tract. Sox17 and Cxcr4 are useful markers of the DE. Previously, we identified a novel DE marker, Decay accelerating factor 1(Daf1/CD55), by identifying DE specific genes from the expression profile of DE derived from mouse embryonic stem cells (ESCs) by microarray analysis, and in situ hybridization of early embryos. Daf1 is expressed in a subpopulation of E-cadherin + Cxcr4+ DE cells. The characteristics of the Daf1-expressing cells during DE differentiation has not been examined.ResultsIn this report, we utilized the ESC differentiation system to examine the characteristics of Daf1-expressing DE cells. We found that Daf1 expression could discriminate late DE from early DE. Early DE cells are Daf1-negative (DE-) and late DE cells are Daf1-positive (DE+). We also found that Daf1+ late DE cells show low proliferative and low cell matrix adhesive characteristics. Furthermore, the purified SOX17low early DE cells gave rise to Daf1+ Sox17high late DE cells.ConclusionDaf1-expressing late definitive endoderm proliferates slowly and show low adhesive capacity.Electronic supplementary materialThe online version of this article (doi:10.1186/s12861-016-0120-2) contains supplementary material, which is available to authorized users.
Highlights
Definitive endoderm (DE) gives rise to the respiratory apparatus and digestive tract
Forkhead box A2 (Foxa2) was highly expressed in the anterior primitive streak (APS) and definitive endoderm (DE)
We analyzed Daf1+ cells for the expression of Nanog, a pluripotent marker, using a mouse Nanog-iPS cell line bearing a green fluorescent protein (GFP) reporter driven under Nanog promoter
Summary
Definitive endoderm (DE) gives rise to the respiratory apparatus and digestive tract. Sox and Cxcr are useful markers of the DE. We identified a novel DE marker, Decay accelerating factor 1(Daf1/CD55), by identifying DE specific genes from the expression profile of DE derived from mouse embryonic stem cells (ESCs) by microarray analysis, and in situ hybridization of early embryos. The definitive endoderm (DE) gives rise to the gastrointestinal and digestive system. The DE progenitors reside at the posterior region of the epiblast that derived from the inner cell mass [1]. The DE is identified by the expression of SRY (sex-determining region Y)-box 17 (Sox17) [7,8,9] and chemokine (C-X-C motif ) receptor 4 (Cxcr4) [10,11,12].
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