Abstract
Laser Tweezers Raman Spectroscopy (LTRS) technology combines Raman spectroscopy and optical tweezers technology, providing a new way to research in single cell analysis and microbial classification. Driven by the development of precision spectroscopy and deep learning techniques, LTRS plays an increasingly important role in biomedical research, clinical diagnosis and environmental research. For example, it can be used to detect cancer cells, analyse bacterial infections, and monitor harmful environmental substances. In this paper, we review the basic principles of LTRS technology through literature reading and case studies, explain how the technology combines the advantages of optical tweezers and Raman spectroscopy in order to provide a non-destructive, label-free and susceptible single-cell analysis method, summarize the latest development of LTRS technology in recent years, as well as its applications in the field of biochemical sensing, and finally discuss the problems it is currently facing and look forward to its future development direction. Finally, the current problems faced by LTRS are discussed, and the direction of its future development is anticipated.
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