Laser Irradiation Alters the Expression Profile of Genes Involved in the Extracellular MatrixIn Vitro

Sandra M Ayuk,Heidi Abrahamse,Nicolette N Houreld

doi:10.1155/2014/604518

Sandra M Ayuk, Heidi Abrahamse + Show 1 more

Open Access

https://doi.org/10.1155/2014/604518

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Abstract

The extracellular matrix (ECM) forms the basis of every phase in wound healing. Healing may be impaired if some of these components are destroyed. Photobiostimulation has demonstrated a stimulatory response in biological processes. This study aimed to evaluate various genes involved in the ECM, in response to laser irradiation. Isolated human skin fibroblasts were used in three different cell models, namely, normal, normal wounded, and diabetic wounded. Cells were irradiated with 5 J/cm2using a continuous wave diode laser emitting at a wavelength of 660 nm and incubated for 48 h. Nonirradiated (0 J/cm2) normal and diabetic wounded cells served as the control. Real-time reverse transcription (RT) quantitative polymerase chain reaction (qPCR) was used to determine the expression of 84 genes in a PCR array. There was a significant upregulation of 29 genes in the normal cells, 32 genes in the normal wounded cells, and 18 genes in the diabetic wounded cells as well as a downregulation of 19 genes (normal), 6 genes (normal wounded), and 31 genes (diabetic wounded). Low intensity laser irradiation (LILI) stimulates gene expression in various cell adhesion molecules (CAMs) and extracellular proteins at 660 nm in wounded fibroblastsin vitro.

Highlights

Components of the extracellular matrix (ECM) have been shown to be useful in wound healing [1, 2]
Due to previous studies which showed an increase in collagen type I (Col-I) in response to laser irradiation at 660 nm [20], this study aimed to determine the effect of laser irradiation at 660 nm on the gene expression profile of the ECM and its cell adhesion molecules
ECM components are very useful in different aspects of wound healing

Summary

Introduction

Components of the extracellular matrix (ECM) have been shown to be useful in wound healing [1, 2]. Venous leg ulcers (VLUs) are amongst the major problems in public health and have become an economic burden in most health care services It is commonly associated with pain, reduces the quality of life, and is even associated with death. COL11A1, COL12A1, COL14A1, COL15A1, COL16A1, COL1A1, COL4A2, COL5A1, COL6A1, COL6A2, COL7A1, COL8A1, FN1, and KAL1 ADAMTS1, ADAMTS13, ADAMTS8, MMP1, MMP10, MMP11, MMP12, MMP13, MMP14, MMP15, MMP16, MMP2, MMP3, MMP7, MMP8, MMP9, SPG7, and TIMP1 COL7A1, KAL1, THBS1, TIMP1, TIMP2, and TIMP3 VCAN, CTGF, ECM1, HAS1, SPP1, TGFBI, THBS2, THBS3, CLEC3B, TNC, and VTN wounds through various cellular or biological processes It is effective in the visible and near infrared (NIR) spectral range. Due to previous studies which showed an increase in collagen type I (Col-I) in response to laser irradiation at 660 nm [20], this study aimed to determine the effect of laser irradiation at 660 nm on the gene expression profile of the ECM and its cell adhesion molecules

Methodology

Results

Discussion and Conclusion