Laser doppler flowmetry microcirculation assessment of a periapical cyst

Abstract

The radicular cyst develops by necrosis in the center of a granulation lesion and expands by applying pressure to the surrounding tissues thus decreasing microcirculation in bone. Laser doppler flowmetry is a non-invasive method of measuring microcirculatory blood flow in tissue. Using laser doppler flowmetry Moor VMS-LDF1-HP and CP1T-HP probe combined with Moor VMS-PC software the microcirculation of the periapical lesion (cyst) was evaluated before surgical endodontic treatment. It was compared to a healthy endodontically treated tooth of the same type with no periapical lesions. Measurements were done with the help of a VMSLDF1-HP probe. Later the bony crypt of the cyst was evaluated for blood perfusions with the VP7BS-HP bone probeEvaluate tissue microcirculation of a periapical lesion before, after and during surgical endodontic treatmentLaser Doppler flowmetry shows that the cystic tooth has a decreased blood flow, decreased concentration, direct current, speed, and lower temperature compared to another endodontically treated lesion-free tooth of the same type. During periapical surgery, the direct laser blood flow evaluation of the surgical crypt shows different values of flux, speed, direct current, and concentration before and after cyst removal which could be attributed to the mechanical trauma (osmotic pressure), adrenaline in the local anaesthetic or laser irradiation of tissues.Laser Doppler flowmetry is a valuable method to perform tissue evaluation before, during and after treatment. It allows us to follow up on the healing and pathological dynamics of microcirculatory tissue changes as well as evaluate and compare different methods and materials for the treatment of apical periodontitis.