Abstract
The method of laser correlation spectroscopy (LCS) is based on the analysis of the spectrum of quasielastic light scatter during coherent monochromatic laser irradiation of micro-particles in biological fluids (blood serum, urine, oropharyngeal washout fluid, tear fluid etc.). Spectrum provides information on dynamic processes in the analyzed system: translation motion of scattering particles and their orientation and conformation dynamics. Special procedures of cluster analysis make it possible to find out to which linkage group a particular spectrum belongs. LCS allows evaluation of sub-fractional composition of biological fluids in a wide range of molecular sizes (from 1 to 10,000 nm), which determines principal novelty of this approach in ophthalmology.
Highlights
The analysis of tear fluid is a simple, safe and minimally invasive method of investigation
In patients after refractive surgery there was marked prevalence in washouts of tear fluid albumin and globulins, which suggested a tendency to catabolic processes which can be caused by increased activity of hydrolases and due to chronic traumatization of the contact lens (CL) and the damaging influence of the laser directly on the collagen fibres of the cornea, destroyed during ablation, small fragments that give this prevalence in the spectrum of the tear fluid
In the samples of Tear fluid patients after PRK slightly predominated small-molecule when it was compared with samples of patients after Trans PRK (13% and 10%, respectively), which may be associated with a higher precision calculation of the ablation zone during the second type of operation
Summary
The analysis of tear fluid is a simple, safe and minimally invasive method of investigation. The recorded spectrum of tear fluid will contribute to increased light scatter low-molecular ingredients - from 10 to 200 nm (mainly albumin and globulins). In patients after refractive surgery there was marked prevalence in washouts of tear fluid albumin and globulins, which suggested a tendency to catabolic processes which can be caused by increased activity of hydrolases and due to chronic traumatization of the contact lens (CL) and the damaging influence of the laser directly on the collagen fibres of the cornea, destroyed during ablation, small fragments that give this prevalence in the spectrum of the tear fluid. The results of this study can be used for screening for monitoring and forecasting process in the postoperative corneal surface ablation [12, 13]
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