Interaction of Titanium Dioxide Nanoparticles and Blood Serum of Patients with Bronchial Asthma and Myasthenia Gravis

Abstract

The interest to nanoobjects is mainly attracted by the fact that their properties (and also biological in particular interest to the project) differ drastically from the properties of either bulk material or constituent molecules. The fundamental task is correlation between structure of the nanoparticles and their interaction with surrounding media. The aim of project is a study of interaction of titanium dioxide nanoparticles and blood serum of patients with verified myasthenia and allergy. Laser correlation spectroscopy (LCS) allows fast and easy hydrodynamic diameter determination in a broad range from nanometer to hundred micrometers, which is an advantage in comparison to other technique for a mixture of nano and microobjects of very different sizes, which constitutes biological liquids. LCS measurement of titanium dioxide nanoparticles specimen showed that main part of light scattering is due to particles with radial size 4.64-8.42 nm. LCS and other physical methods measurement data are coincided. Comparison of LC-histograms of blood serum revealed increased contribution of 4.64 -15.28 nm particles into light scattering in bronchial asthma patients and 404.65 – 1794.35 nm particles in myasthenia gravis patients. Interaction between titanium dioxide nanoparticles, stabilized by phosphoric acid, and blood serum results to albumin- and globulin-containing complexes formation and precipitation. Interaction between titanium dioxide nanoparticles, stabilized by phosphoric acid, and blood serum results to albumin- and globulin-containing complexes formation and precipitation. Interaction between titanium dioxide nanoparticles and myasthenia patients blood serum results to removing of considerable part (40%) of circulating immune complexes. TiO2 nanoparticles under interaction with asthma patients blood serum form complexes with small size proteins. LCS analysis confirmed removal of particles corresponding by their size to the major serum proteins and redistribution towards larger particles presented by glycolipids, glycoproteins, and antigen-antibody complexes.