Larvicidal and ovicidal activity of Terminalia chebula Retz. (Family: Combretaceae) medicinal plant extracts against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus.

Abstract

Insect-borne diseases remain to this day a major source of illness and death worldwide. The resistance to chemical insecticides among mosquito species has been considered as a setback in vector control. Mosquito control programs, botanical origin may have the potential to eliminate eggs and larvae. So, the larvicidal and ovicidal activities of crude benzene, hexane, ethyl acetate, chloroform and methanol extracts of Terminalia chebula were assayed for their toxicity against three important vector mosquitoes, viz., Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus. The larval mortality was observed after 24h of exposure. All extracts showed moderate larvicidal effects; however, the highest larval mortality was found in the methanol extract of T. chebula against the larvae of A. stephensi, A. aegypti, and C. quinquefasciatus with the LC50 values were 87.13, 93.24 and 111.98ppm, respectively. Mean percent hatchability of the ovicidal activity was observed 48h post treatment. The percent hatchability was inversely proportional to the concentration of extract and directly proportional to the eggs. All the five solvent extracts showed moderate ovicidal activity; however, the maximum egg mortality (zero hatchability) was observed in the methanol extract of T. chebula at 200 and 250ppm against A. stephensi, A. aegypti and C. quinquefasciatus showed 100% mortality at 300ppm. No mortality was recorded in the control. The finding of the present investigation revealed that the leaf extract of Terminalia chebula possesses remarkable larvicidal and ovicidal activity against medically important vector mosquitoes and make this plant product promising as an alternative to synthetic insecticide in mosquito control programs.