Abstract
Systematic study of cellular interactions in a 2D tissue culture requires specific control of a set of ligands relevant for the particular interaction. Many cellular- and signaling interactions happen at the nanoscale, and a nanopatterning toolkit can shed more light on these interactions. Multi-ligand nanopatterning approaches, such as dip-pen nanolithography and DNA origami nanoarrays, are currently available for multivalency studies in biological systems. However, dip-pen nanolithography is slow and requires specialized instruments, while the DNA origami-based approaches are restricted in dimensions, highly negatively charged and unstable in physiological conditions.
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