Abstract
Abstract The brain and dendritic cell (BAD)-associated lysosome-associated membrane protein (LAMP)-like molecule, also known as BAD-LAMP, c20orf103, UNC-46, is a newly identified LAMP family member, which has been recently officially designated as LAMP5. It is a transmembrane glycosylated protein localized in the ER-Golgi intermediate compartment (ERGIC). LAMP5 shares sequence and structural homology with other LAMP family members. In mouse, LAMP5 is expressed in postnatal cortical neurons of particular layers, where it is enriched in defined zones along neuronal projections. In human, like its murine homolog, LAMP5 is principally expressed in brain, and it was recently found in primary plasmacytoid dendritic cells (pDCs). However, LAMP5 expression profile and function have not been extensively studied in pDCs. By using anti-human LAMP5 monoclonal antibody (clone 124-40B), we report that LAMP5 is specifically expressed in peripheral blood pDCs which are characterized by surface expression of CD303+/CD304+/CD123+/HLA-DR+/CXCR3+/CD11c-/CD14-/CD209-, but is not expressed by GM-CSF plus FL3 or IL-4 induced monocyte-derived CD303+/CD123+/HLA-DR+/CD209+/CD14+/CXCR3dim pDCs or CD209+/CD123dim/CXCR3dim/HLA-DR+/CD11c+/CD14+ mDCs. Like other LAMP members, LAMP5 is rapidly relocated on the cell surface of peripheral blood pDCs after 24 hours stimulation by IL-3. These data suggest that LAMP5 may be a useful marker for studying pDC development and activation.
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