Abstract
Laminarin, a β-(1,3)-glucan from the seaweed Laminaria digitata, is a polysaccharide which provides anti-inflammatory and anti-oxidative properties. Its influence on both human dermal fibroblasts adult (HDFa) and normal human epidermal keratinocytes (NHEK) has not been established yet. Herein, laminarin effects were examined on skin cells’ mitochondrial and antioxidant activities. Cytokines, hyaluronic acid, and procollagen type I secretions and interaction mechanisms were explored after a maximum of 72 h treatment with laminarin. Our results demonstrated a decrease in mitochondrial activities with 72 h treatment with laminarin from 500 µg.mL−1 for NHEK cells and from 100 µg.mL−1 for HDFa cells without cytotoxicity. No variation of hyaluronic acid or type I procollagen was observed for all laminarin concentrations, while an antioxidant effect was found against reactive oxygen species (ROS) from 1 µg.mL−1 for HDFa cells in both H2O2 and UVA radiation conditions, and from 10 µg.mL−1 and 1 µg.mL−1 for NHEK cells in both H2O2 and UVA radiation conditions, respectively. Laminarin treatment modulated both cells surface glycosylation and cytokine secretions of skin cells. Overall, our data suggest a positive effect of β-(1,3)-glucan on skin cells on oxidative stress and inflammation induced by environmental factors. Of note, these effects are through the modulation of glycan and receptors interactions at the skin cells surface.
Highlights
Research has focused on β-glucans, polysaccharides that are widely present in the cell walls of bacteria, algae, fungi, yeasts, or cereals [1,2,3], for their physico-chemical properties such as thickening, stabilizing, emulsification, and gelation [4]
The effect of laminarin was evaluated at several concentrations (1, 10, 100, 500, and 1000 μg.mL−1 ) in human dermal fibroblasts adult (HDFa), normal human epidermal keratinocytes (NHEK), and HaCaT cells at 24 h, 48 h, and 72 h
Regarding laminarin effects on cells, our results showed that laminarin induced a significant decrease in cell metabolic activity in a time- and concentration-dependent manner from 100 μg.mL−1 for HDFa and 500 μg.mL−1 for NHEK cells
Summary
Research has focused on β-glucans, polysaccharides that are widely present in the cell walls of bacteria, algae, fungi, yeasts, or cereals [1,2,3], for their physico-chemical properties such as thickening, stabilizing, emulsification, and gelation [4]. Β-glucans structure such as linear β-(1→3)- or β-(1→6)-chain core, molecular weight, length, or the number and degree of branching side-chain influence their solubility and conformation in aqueous media (single helix, triple helix, random coil conformation) [5]. These structural characteristics confer β-glucans biological properties, such as anti-cancer [6,7], anti-oxidative [8], and anti-inflammatory effects [9]. Β-glucans are used in cosmetics [1] for their antioxidant and moisturizing properties [14,15], and as a texturizing agent in cosmetic creams [16]
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call