Abstract
We report transcription in vitro of the lambda repressor gene, cI, using specific restriction endonuclease fragments as templates. This transcription is repressed by lambda repressor. Moreover, we report the sequence change caused by a cI promoter mutation. This change is located between two repressor binding sites in the rightward operator (OR). Transcription studies using mutant templates indicate that repressor bound to two sites in OR regulates transcription of gene tof, and repressor bound to the remaining site(s) controls transcription of cI.
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