Lactobacillus plantarum LAB12 Enhances Cognitive Function via Modulation of Neurotransmitters and Neuropeptides in Antibiotic- and LPS-challenged Rats

Abstract

Event Abstract Back to Event Lactobacillus plantarum LAB12 enhances cognitive function via modulation of neurotransmitters and neuropeptides in antibiotic- and LPS-challenged rats Muhammad Syukri Noor Azman1, 2, Siong Meng Lim1, 2, Fei Tieng Lim2 and Kalavathy Ramasamy1, 2* 1 Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Malaysia 2 Collaborative Drug Discovery Research (CDDR) Group, Universiti Teknologi MARA (UiTM), Pharmaceutical and Life Sciences Community of Research, Malaysia Background Based on the notion that probiotic lactic acid bacteria (LAB) could potentially correct dysbiosis of microbiota and ameliorate neuroinflammation via the Gut-Brain Axis, this study assessed the effects of LAB12 on memory, expression of major neurotransmitters and neuropeptides. Methods Sprague Dawley rats (male, 3 months old) were being administered with an antibiotic cocktail (imipenem, vancomycin, ampicillin, ciprofloxacin and metronidazole) before being challenged with 0.25mg/kg LPS. The rodents were divided into four groups (n=6/group): wild-type, LPS control, antibiotics (ABX) +LPS and ABX+LAB12+LPS. The rats were subjected to the Morris Water Maze Test. Rat hippocampi were harvested and subjected to biochemical analyses and metabolic assays. Results LAB12 improved spatial learning and memory in antibiotic- and LPS-challenged rats. ABX+LAB12+LPS group was presented with reduced escape latency and escape distance when compared to the ABX+LPS counterpart. In terms of neurotransmitters in the hippocampus, increased serotonin (5HT) and gamma-aminobutyric acid (GABA) was observed in ABX+LAB12+LPS when compared to ABX+LPS. There were also increased neurotensin (NT) and neuropeptide Y (NPY) levels in the hippocampus of ABX+LAB12+LPS when compared to ABX+LPS, indicating enhanced cognitive processing. Whilst mitochondrial complex enzymes (II, III and IV) were impaired in the hippocampus of ABX+LPS rats, supplementation of LAB12 ameliorated this effect. Conclusion The present findings implied that L. plantarum LAB12 could potentially improve memory impairment via modulation of neurotransmitters and neuropeptides in the CNS. Keywords: Lactobacillus plantarum LAB12, Memory enhancing, neurotransmitters, Neuropeptides, antibiotics Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Mental health Citation: Noor Azman M, Lim S, Lim F and Ramasamy K (2019). Lactobacillus plantarum LAB12 enhances cognitive function via modulation of neurotransmitters and neuropeptides in antibiotic- and LPS-challenged rats. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00074 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Nov 2018; Published Online: 17 Jan 2019. * Correspondence: PhD. Kalavathy Ramasamy, Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Bandar Puncak Alam, Selangor Darul Ehsan, Malaysia, kalav922@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Muhammad Syukri Noor Azman Siong Meng Lim Fei Tieng Lim Kalavathy Ramasamy Google Muhammad Syukri Noor Azman Siong Meng Lim Fei Tieng Lim Kalavathy Ramasamy Google Scholar Muhammad Syukri Noor Azman Siong Meng Lim Fei Tieng Lim Kalavathy Ramasamy PubMed Muhammad Syukri Noor Azman Siong Meng Lim Fei Tieng Lim Kalavathy Ramasamy Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.