Chemical and molecular diversity analysis of Curcuma longa L. across the Indian subcontinent

Abstract

Event Abstract Back to Event Chemical and molecular diversity analysis of Curcuma longa L. across the Indian subcontinent KAMRAN ASHRAF1, 2, 3* and Mohammad Mujeeb3 1 Universiti Teknologi MARA Puncak Alam, Department of Pharmacology & Chemistry, Faculty of Pharmacy, Malaysia 2 Atta-ur-Rahman Institute for Natural Product Discovey, Universiti Teknologi MARA, Malaysia 3 Jamia Hamdard University, Bioactive Natural Product Laboratory, School of Pharmaceutical Education & Research, India Background Curcuma longa (turmeric) is a valued medicinal plant belonging to the family Zingiberaceae which comprises more than 80 species of rhizomatous perennial herbs and has extensive occurrence in the tropics of Asia, Africa and Australia. Previous studies of turmeric did not show any data of molecular and chemo profiling side by side. As this study could generate good information for better reproducibility and cultivation of this plant. So the aim of the research was to find out chemical and molecular diversity among accessions of turmeric collected across the Indian subcontinent. Methods Phytochemical constituent curcuminoids of eight accessions of turmeric were evaluated by ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS/MS) method. Molecular diversity analysis was done by polymerase chain reaction (PCR) based random amplification polymorphism DNA(RAPD) method. Results Results of chemo profiling by ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS/MS) showed large variations in the content of curcuminoids (1.408 – 5.027% w/w) and RAPD based molecular diversity analysis also showed excellent genetic variations (44.44% to 100%) among samples of C. longa. Results also showed that Erode (southern part) of India produced higher yield of curcuminoids (50.27 mg/g). These results revealed that C. longa has undergone both chemical as well as genetic variations. Conclusion From these results it is concluded that chemical and molecular diversity occurred in C. longa and Erode (southern province) contains the highest quantity of curcuminoids. Erode variety could be superior and can be used for large scale production and cultivation. These variations in turmeric may be due to wide range of ecological conditions within distribution area of its population in India. These outcomes would provide an important input into determining efficient management strategies for the cultivation and improvement program of C. longaglobally and in India in particular. Acknowledgements Kamran Ashraf would like to acknowledge Universiti Teknologi MARA for the financial support under the reference number 600-IRMI/MyRA 5/3/LESTARI (079/2017). Authors also would like to acknowledge the University Grand Commission, Government of India for the financial support. Keywords: Curcuma longa, turmeric, UPLC-MS/MS, RAPD, India Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Oral Presentation Topic: Miscellaneous Citation: ASHRAF K and Mujeeb M (2019). Chemical and molecular diversity analysis of Curcuma longa L. across the Indian subcontinent. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00039 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 24 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. KAMRAN ASHRAF, Universiti Teknologi MARA Puncak Alam, Department of Pharmacology & Chemistry, Faculty of Pharmacy, Selangor, Malaysia, kamran1368@puncakalam.uitm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. 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