Lactobacillus gasseri enhance gut homeostasis in normal mice and alleviate symptoms in Salmonella typhimurium-infected mice via bacteriocin generation

Abstract

Bacteriocins are a class of anti-microbial proteins or peptides produced by bacteria that have antimicrobial activity and are regarded as potential alternatives to antibiotics. Lactobacillus (L.) gasseri, which is a probiotic, can produce bacteriocins. In the present study, we determined the bacteriostatic ability and bacteriocin-producing species of four strains of L. gasseri and investigated the effects of gavage of different bacteriocin-producing L. gasseri, on serum biochemical indices, intestinal cytokines, and gut microbiota of normal mice, a total of 40 C57BL/6J mice were used in the above study, where the gavage L. gasseri group was given a dose of 109 CFU/d. The ability of different bacteriocin-producing L. gasseri, to alleviate the symptoms caused by Salmonella (S.) typhimurium infection was further investigated, and the key bacteriocins and potential mechanisms were discuss, a total of 48 C57BL/6J mice were used in the above study.The results showed that Helveticin J, Gassericin K7B, Acidocin B and Bacteriocin LS2-producing L. gasseri FJXPY47L6, and FGSYC2L2, and FHNFQ28L4 could significantly improve the symptoms caused by S. typhimurium infection, and the bacteriocin-producing L. gasseri FJXPY47L6, and FGSYC2L2. Bacteriocin LS2-producing FJXPY47L6 showed the greatest ability to alleviate symptoms caused by S. typhimurium infection. These effects are achieved through bacteriocin-producing modulation of intestinal cytokines, gut microbiota, and short-chain fatty acids.