Lactobacilli and bifidobacteria lectins as possible signal molecules regulating intra- and inter-population bacteria–bacteria and host–bacteria relationships. Part I. Methods of bacterial lectin isolation, physico-chemical characterization and some biological activity investigation

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Lectins (natural or artificial nonimmunoglobulin origin proteins/glycoproteins) are able to bind noncovalently with carbohydrate targets. Lectins and lectin-like substances of lactobacilli (L. acidophilus K3III24; 100ash; NK1; L. plantarum 8RA3) and bifidobacteria (B. adolescentis MC42; B gallinarum, B. bifidum 1) have been investigated. Bacteria were grown in semi-anaerobic conditions in a casein-yeast broth. Medium was microfiltrated and ultrafiltrated for isolation of 30 kDa compounds which were then separated by isoelectric focusing in polyacrylamide gel in the presence of urea and sucrose. Components isolated were electrotransferred to immobillon P. Blots obtained were then treated with a set of biotinylated artificial linear homopolysaccharides revealed by streptavidin-peroxidase conjugate. Chemiluminescence registration of peroxidase bound to blots was performed using the ‘BioChemi System’ camera. A spectrum of different polysaccharide-binding individual components reacting with such homopolymers a...