Lactic acid bacteria inhibits quorum sensing and biofilm formation of Pseudomonas aeruginosa strain JUPG01 isolated from rancid butter

Abstract

The majority of pathogenic bacteria utilize cell to cell communication mechanisms to regulate the cell density and expression of virulence factors. Therefore, quorum sensing system could be one of the targets for antimicrobial strategy to control quorum sensing regulated phenotypes in the pathogenic bacteria. Several lactic acid bacteria produce peptides and secondary metabolites having antimicrobial properties. In the present study, exopolysaccharides (EPS) and secondary metabolites from the Lactic Acid Bacteria (LABs) isolated from various sources such as cow milk, sourdough, chicken intestine, buttermilk, roasted meat and idly batter were screened for quorum quenching properties against P. aeruginosa strain JUPG01isolated from rancid butter. The results revealed that EPS from LABs were not significant against screened quorum quenching activity. However, the secondary metabolites extracted from Pediococcus pentosaceus strain BS-2 and Lactobacillus fermentum strain BM-2isolated from cow milk exhibited significant quorum quenching activity. Furthermore, the strains BS-2 and BM-2 revealed significant anti-biofilm activity against P. aeruginosa strain JUPG01. This study highlights the biotechnological applications of LABs in the eradication of Pseudomonas species associated with dairy food contamination and spoilage.