Abstract
All starch digesting activities are reported absent in suckling mice by jejunal mucosal enzyme assay, imunohistochemistry or Western blots. We confirmed that there was no maltase activity or brush border staining with Mgam antibodies at 13d and that these were detected at 25d only in WT. Last year we reported that at 13 d both Mgam null and WT suckling mice can digest amylase treated 13C‐starch derived α‐limit dextrins (LDx) to increase blood 13C glucose paradoxically in 13d Mgam null suckling mice lumenal contents were stained by specific Mgam antibodies.ObjectiveTest lactating mouse mammary glands for Mgam and Si gene expression.MethodsRNA was extracted from the mammary gland of 10 different strains of WT 30d pp lactating mice. After RT, the cDNA was amplified for Mouse Si (amplicons for exons 1 to 6 or exons 16 to 18) and Mgam (amplicons for exons 1 to 7 or exons 3 to 7).ResultsOnly the Mgam Exon 3 to 7 primers produced 516 bp bands on the Gel.Conclusionwe suggest that maltase activity is secreted in mouse milk which enables unweaned pup starch digestion well before brush border bound enzyme development. Splicoforms of the Mgam message, lacking the membrane binding exon 2 coding region, in lactating mouse mammary tissue suggest synthesis of a soluble enzyme activity. This was confirmed by the expression of the exon 2 deleted Mgam message in lactating mammary glands of 10 different mouse strains.
Published Version
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