Abstract
Laccase has the ability to act on a broad range of substrates, as a result of which multiple compounds have been used for laccase assay and numerous applications have been discovered over time. Thus, considering the above points, in the present study, white and blue laccase from Myrothecium verrucaria ITCC-8447 and Pleurotus ostreatus were used for laccase assay using various substrates which includes 2,2′ azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,6-dimethoxyphenol (2, 6 DMP), guaiacol (GCL), and syringaldazine (SYZ). The most effective substrate of the four substrates was ABTS for white and blue laccase. The white and blue laccase were further used for the synthesis of bio-material via fungal-assisted treatment of wheat bran (WB) and sugarcane bagasse (SB) followed by micro-wave pre-treatment and the addition of starch and glycerol to the treated agro-residue in the ratio of 5:1:1. The thickness swelling and water absorption percentage was minimum for wheat bran synthesized bio-material using white laccase in comparison to the other synthesized bio-material.
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