Lacaune ewes with subclinical mastitis: effects of intramammary application of própolis

Guilherme Luiz Deolindo,Vitor Luiz Molosse,Lilian Kolling Girardini,Denise Nunes Araujo,Marcelo Vedovatto,Aleksandro Schafer Da Silva,Amanda Dilda

doi:10.33448/rsd-v10i2.11709

Abstract

The aim of this study was to evaluate the antimicrobial potential of the aqueous extract of green propolis in vitro against mastitis-causing and in vivo bacteria, evaluating the efficacy of treatment in Lacaune sheep. In the in vitro test, the minimum inhibitory concentration (MIC) was used; first, the MIC was obtained from the aqueous extract of green propolis for the strain of Staphylococcus. aureus ATCC 25523, defined as 1 mg/ml. For bacterial agents isolated from sheep with mastitis (Staphylococcus epidermidis, Staphylococcus intermedius, Staphylococcus hyicus, Corynebacterium spp. and Acinetobacter spp.), The concentration of 10 mg/ml was determined, while for Streptococcus equinus, Escherichia coli, and hemolytic E. coli, also isolated from sheep with mastitis, the concentration capable of reducing bacterial growth was 100 mg/ml. In the in vivo test, ten sheep were used, distributed in two treatments, five in the control group (CG) that received 2.5 ml of saline (vehicle), and five in the treated group (GT) that received 2.5 ml of aqueous (saline) propolis extract by the mammary route. The propolis dose tested (0.1 g/ml) was not effective for the treatment of mastitis, because the sheep remained positive in the racket test (CMT). The main microorganism isolated in the cases of mastitis in this study was Staphylococcus epidermidis. These results are preliminary; however, at the tested dose, the aqueous extract of green propolis delivered by the mammary route had no curative effect of mastitis.

Highlights

In the Brazilian livestock industry, sheep farming is vying for a more prominent presence in the dairy sector (Rohenkohl et al, 2011)
Because of the great relevance that mastitis has in the sheep milk production chain, in the present study, we aimed to evaluate the antimicrobial potential of aqueous green propolis extract in vitro against mastitis-causing bacteria and in vivo, evaluating the effectiveness of treatment in sheep from the Lacaune breed
From the milk samples collected, we obtained bacterial growth of Staphylococcus epidermidis (n = 29, corresponding to 46% of the isolated agents), Staphylococcus hyicus (n = 11, corresponding to 17% of the isolated agents), Escherichia coli (n = 10, corresponding to 16% of the isolated agents), hemolytic E. coli (n = 5, corresponding to 8% of the isolated agents), Staphylococcus intermedius (n = 4, corresponding to 6% of the isolated agents), Corynebacterium spp. (n = 2, corresponding to 3% of the isolated agents), Acinetobacter spp. (n = 1, corresponding to 2% of the isolated agents), yeast (n = 1, corresponding to 2% of the isolated agents), and Streptococcus equinus (n = 1, corresponding to 2% of the isolated agents) (Table 1)

Summary

Introduction

In the Brazilian livestock industry, sheep farming is vying for a more prominent presence in the dairy sector (Rohenkohl et al, 2011). Milk is a vital food and a nutritional supplementation deployed in the fight against hunger (Haenlein, 2001) This is because, when compared with bovine milk, sheep's milk has higher levels of protein, calcium, and phosphorus, as well as high-quality fat [Souza et al, 2001; Revers et al, 2016). Mastitis directly affects milk production and quality, resulting in changes in milk composition (increased somatic cell counts and reduced fat and protein levels) resulting from inflammation Rovai et al, 2015) This phenomenon affects the industry as a whole as well as individual producers, changing the sensory characteristics of the final product and causing economic losses for dairy farms

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