Abstract
The in vivo methods presently used for testing rabies vaccine and rabies antibody are generally considered quite variable. This variability can be measured by each laboratory and can be controlled by the use of appropriate reference preparations. The NIH potency test and the antibody-binding test for rabies vaccine are compared as well as the mouse neutralization test (MNT) and rapid fluorescent focus inhibition test (RFFIT) for rabies antibody. The antibody assay in immunized mice is presented as a new method for testing the potency of rabies vaccines. The concept of a ‘unit ratio’ is introduced as an indicator of vaccine potency and this is compared to the antigenic value generated by the more conventional testing methods.
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