Laboratory bioassay of some entomopathogenic fungi on Spodoptera littoralis (Boisd.) and Agrotis ipsilon (Hufn.) larvae (Lepidoptera: Noctuidae).

Abstract

Efficacy of entomopathogenic fungi products (i.e. Bio- Power (Beauveria bassiana), Bio-Catch (Lecanicillium lecanii) and Priority ( Paecilomyces fumosoroseus)) against Spodoptera littoralis and Agrotis ipsilon larvae were evaluated under laboratory conditions. Four different concentrations i.e. 0.125x10 9 , 0.25x10 9 , 0.5x10 9 and 1x10 9 spores/ 1000ml D.W of each formulation were used against each pest under investigation and compared with control insects. The results obtained show that Bio- Power was the most effective product followed by Bio-Catch and Priority against S. littoralis 3 rd instar larvae, whereas , the LC50,90 values were 0.2x10 9 and 1.5x10 9 , 0.22x10 9 and 4.6x10 9 , 0.44x10 9 and 4.7x10 9 , respectively. While in case of A. ipsilon, Priority was the most potent product followed by Bio- Power and BioCatch, whereas, the LC50,90 values were 0.14x10 9 and 0.6x10 9 , 0.2x10 9 and 0.9x10 9 , 0.4x10 9 and 9.7x10 9 , respectively. Also, The results obtained show that percentage mortalities of treated third instar larvae of S. littoralis with Bio- Power, Bio-Catch and Priority were 87.5,72.5 and 67.5 %, respectively ,within 6.3,8.2 and 7.4 days at the highest concentration used (1x10 9 spores). However, The treated third instar larvae of A. ipsilon with Priority, Bio- Power and Bio-Catch achieved 100, 90.0 and 62.5 % mortality, respectively, within 5.3, 7.1and 3.9 days at concentration of 1x10 9 spores. The different formulations had delayed effects, the percentage of pupae and adults emerged were significantly decreased with an increase in concentrations.