Abstract
Purpose Indirect immunofluorescence (IIF) in Hep-2 cells is the standard screening test for antinuclear antibodies (ANA). Advances in this methodology have brought up a considerable increase in sensitivity and consequently a decrease in its specificity. This has resulted in an increasing number of positive tests in apparently healthy subjects. In particular, autoantibodies associated with the dense fine speckled (DSF) ANA-Hep-2 pattern, has been largely detected in apparently healthy individuals.
Highlights
Indirect immunofluorescence (IIF) in Hep-2 cells is the standard screening test for antinuclear antibodies (ANA)
ANA-Hep-2 test from 921 children aged 0.2 to 13.9 years was retrospectively retrieved from the routine of a large private laboratory on-site certified by the College of American Pathologists (CAP)
The DFS pattern was a frequent finding in the ANAHep-2 routine and its occurrence was not associated with abnormalities in general laboratory tests
Summary
Laboratorial significance of autoantibodies of dense fine speckled pattern Tania Caroline Castro*, Daniela Petry Piotto. From 2011 Pediatric Rheumatology Symposium sponsored by the American College of Rheumatology Miami, FL, USA. Purpose Indirect immunofluorescence (IIF) in Hep-2 cells is the standard screening test for antinuclear antibodies (ANA). Advances in this methodology have brought up a considerable increase in sensitivity and a decrease in its specificity. This has resulted in an increasing number of positive tests in apparently healthy subjects. Autoantibodies associated with the dense fine speckled (DSF) ANA-Hep-2 pattern, has been largely detected in apparently healthy individuals
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