Abstract
In the past two decades, single molecule localization microscopy has revolutionized bioimaging with the superresolution. Through a different approach, a more recent technology Expansion Microscopy elevated the resolution again using expandable hydrogel. This talk will decipher a novel hybrid method, Label-Retention Expansion Microscopy (LR-ExM), which uses trifunctional chemical labels to combine these two microscopy approaches for molecular resolution imaging. This method achieved molecular resolution and outstanding labeling efficiency, which allows visualization of individual proteins, genes, and RNA molecules in situ.
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