Abstract
A method for the 11C-labelling of proteins for use in positron emission tomography is presented. Human iron-free transferrin and human serum albumin were treated with [ 11C]cyanogen bromide of high specific radioactivity under physiological conditions to give the labelled proteins in high radiochemical yields using a simple and rapid procedure. In a typical experiment, 2.0 GBq of [ 11C]albumin was obtained in 28–32 min synthesis time counted from the end of bombardment. The radiochemical purity was in all cases > 98% and the specific radioactivity varied with the amount of protein between 10.7–141 GBq μmol -1 (0.3–3.8 Ci μmol -1). The radiochemical yield of labelled product as a function of pH and protein concentration was studied. The labelled products were characterized using both high performance liquid chromatography and high performance capillary electrophoresis with u.v.- and radiodetection in series.
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