Label-free Kanamycin sensor development based on CuO[sbnd]NiO hollow-spheres: Food samples analyses

Abstract

Kanamycin (KAN) is commonly used as an amino-glycoside antibiotic and enduring KAN in animal derivative food causes intensive adverse effects in human health. Here, at first copper oxide co-doped nickel oxide hollow-spheres (CuONiO HSs) were synthesized using a facile hydrothermal method (HTM) in an alkaline phase and then characterized by various conventional methods such as Fourier-transform infrared spectroscopy (FTIR), UV–visible spectroscopy (UV–vis.), Field Emission Scanning Electron Microscopy (FESEM), Energy-dispersive X-ray spectroscopy (XEDS), X-ray photoelectron spectroscopy (XPS), and powder X-ray diffraction (XRD) to find out the nature of materials as binding energy, band-gap energy, morphology, crystallinity, functional groups, and elemental analyses. A selective and label-free KAN sensor was developed with the modification of slight coating of HSs onto a glassy carbon electrode (GCE) using nafion (Nf) coating binder at room conditions. Improved electrochemical performances of sensitivity, linear dynamic range (LDR), and long-term stability of selective KAN were achieved by reliable electrochemical technique. Based on signal to noise ratio 3, sensitivity and limit of detection (LOD) of sensor were calculated as 1.9 × 10−4 μAμM−1 cm−2 and 0.5 ± 0.02 nM respectively. The hydrothermally synthesized CuONiO HSs is significant advancement in fabricating with Nf/GCE decorated sensor probe in favor of enzyme-free detection of chemicals in biological system. In conclusion, the proposed sensors can be applied effectively for the selective and sensitive detection of KAN in food samples with satisfactory and acceptable results.